Default BLAST parameters for a protein sequence of length longer than 30 amino acids: E value: 10 Word size: 3 Matrix: BLOSUM62 Gap open cost: 11 Gap extention cost: 1 Default BLAST parameters for a short protein sequence of length equal to or less than 30 amino acids: E value: 100 Word size: 2 Matrix: PAM30 Gap open cost: 11 Gap extention cost: 1 |